Method to investigate the effect of temperature on the rate of decay
1. Measure 5 cm³ of lipase into a test tube and label tube
2. Measure 5 cm³ of milk and add to a different test tube
3. Add 5 drops of phenolphthalein to tube containing milk
4. Measure 7 cm³ of sodium carbonate and add to the milk test tube (now alkaline so should turn pink)
5. Put both tube in water bath at 30°C and leave tubes to reach same temperature (use a thermometer in milk test tube)
6. At 30°C, add 1cm³ of lipase solution into the milk and start stopwatch
7. Stir contents and enzyme will start to decompose milk
8. When it loses its pink colour, stop the stopwatch and record time
9. Repeat with different temperatures