Plant tissue culture

• Tissue culture is in vitro cultivation of plant cell or tissue under aseptic and controlled environmental conditions, in liquid or on semisolid well-defined nutrient medium for the production of primary and secondary metabolites or to regenerate plant
• This technique affords alternative solution to problems arising due to current rate of extinction and decimation of flora and ecosystem
• The whole process requires a well-equipped culture laboratory and nutrient medium
• This process involves various steps, viz. preparation of nutrient medium containing inorganic and organic salts, supplemented with vitamins, plant growth hormone(s) and amino acids as well as sterilization of explant (source of plant tissue), glassware and other accessories inoculation and incubation
• Applications:

  • Production of Phytopharmaceuticals
  • Biochemical conversions
  • Clonal propagation (Micro-propagation)
  • Production of Immobilized Plant Cell