1. Remove the gloves and perform hand hygiene
2. Open the sterile dressing set
4. Cleanse the wound using aseptic technique
6. If a topical antimicrobial ointment is applied, wipe and irrigate to remove it
7. Clean the wound with normal saline solution until all exudate has been removed
8. After cleansing apply a sterile gauze pad to the wound
9. Remove gloves and perform hand hygiene
10. Obtaining Aerobic Culture
12. Open the specimen tube and place the cap upside down on a firm, dry surface
13. Rotate the swab back and forth over clean areas of granulation tissue
14. DO NOT USE pus or exudates to culture
15. Avoid touching the swab to intact skin at the wound edges
16. Return the swab to the culture tube
17. Crush the barrier to the inner compartment containing the transport medium
18. If a specimen is required from another site, repeat the steps
19. Obtaining Anaerobic Culture
21. Insert a sterile 10-mL syringe into the wound
22. Attach the needle to the syringe and expel all air
23. Immediately inject the drainage into the anaerobic culture tube
24. Label the tube or syringe appropriately
25. Remove and discard gloves, perform hand hygiene
26. Send the tube or syringe of drainage to the laboratory immediately
27. Dress the wound and document the procedure done along with pertinent information
28. Apply any ordered medication to the wound
29. Cover the wound with sterile dressing
30. Remove gloves and perform hand hygiene