Outline the practical procedure used to measure the effect of enzyme concentration on enzyme activity, using trypsin and milk.
• Dilute stock solution of trypsin with distilled water to produce 4 concentrations.
• Set up a control and use it to set the colorimeter absorbance to zero.
• To another cuvette, add 2 cm 3 of milk suspension and 2 cm 3 of the stock trypsin solution. Mix, place in the colorimeter and measure absorbance at 15 second intervals for 5 minutes.
• Rinse the cuvette with distilled water.
• Repeat step 3 at all trypsin concentrations.