The benedicts test- make a solution of the test materialAdd a roughly equal volume of benedict's solutionheat the solution in a water bath to 80oC for 5 minutes
Acid hydrolysis:Make a solution of test materialadd a few drops of HCL to test solutionHeat in a water bath for a few minutesneutralise the acid with sodium hydrogen carbonatecarry out the benedicts test
How do you carry out a quantative test for glucose?
Carry out the benedicts test on a solution of unknown glucose concentrationcalibrate a colourimeter using a vial of distilled watertest a number of samples of solution of known glucose concentrationrecord the absorbsion/transmittionplot a graph for these resultstest the unknown sampleplot the result and compare to the line of best fit to find the concentration