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B1
microscopy
What is it?
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Cards (25)
What does microscopy refer to?
The use of
microscopes
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What are the main components of a light microscope?
Base, arm,
light source
, stage,
lenses
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What supports the entire light microscope?
The
base
at the bottom
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What is the function of the arm in a microscope?
It connects the
base
to the
top
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What type of light source can be used in a microscope?
A
lamp
or a
mirror
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Where is the stage located on a light microscope?
Just above the
light source
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How many objective lenses are typically found in a light microscope?
Three
objective lenses
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What are the typical magnifications of objective lenses?
10x
,
20x
, and
50x
magnifications
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What is the function of the eyepiece lens?
To look into the
microscope
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What do the coarse and fine focusing knobs do?
Help get the
image
in
focus
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What does the term 'object' refer to in microscopy?
The
real
object
being
observed
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How is the term 'image' defined in microscopy?
The visual representation seen through the
microscope
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How do the terms 'object' and 'image' differ in microscopy?
The object is
real
; the image is
visual
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How does light travel through a light microscope?
It
reflects
through the object and lenses
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What is magnification in microscopy?
How many
times
larger the image is
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What is the equation for magnification?
Magnification =
image size
/
object size
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If an image is 1000 times larger than the object, what is the magnification?
1000
times
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What does resolution refer to in microscopy?
The shortest distance between
distinguishable
points
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How does resolution affect image quality?
Higher resolution means more detail
visible
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What happens to the image if the resolution is low?
The image appears
blurry
and
less
detailed
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What is the relationship between magnification and resolution?
Higher
magnification can lead to
lower
resolution
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What are the key terms related to microscopy?
Object
: The real sample being observed
Image
: The visual representation seen through the microscope
Magnification
: How many times larger the image is than the object
Resolution
: The shortest distance between distinguishable points
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What is the process of light travel in a light microscope?
Light hits the mirror and reflects upwards
Light passes through the object on the stage
Light goes through an
objective lens
Light travels through the
eyepiece lens
Light enters the observer's eye
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How does resolution affect the clarity of images in microscopy?
Higher
resolution allows for more detail
Lower
resolution results in a blurrier image
Resolution is crucial for distinguishing features
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What is the significance of the magnification equation?
Magnification =
image size
/
object size
Helps calculate how much larger the image appears
Essential for understanding
microscope
capabilities
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