• genetically modified bacteria
o a plasmid is removed from a bacteria using lysozyme
o the insulin producing gene is removed from a human chromosome using restriction enzyme
o the plasmid is cut open using the same restriction enzyme – leaving the same sticky end
o the plasmid and human gene are joined using ligase enzyme which joins the sticky ends producing recombinant DNA
o the recombinant DNA is inserted into a bacterium
o the transgenic bacterium is grown in a vat, millions of identical bacterium are collected which all produce human insulin – the insulin is