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Biology Paper 1
Organisation
Food tests (PRACTICAL )
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How do we use chemical tests for carbohydrates,proteins,and lipids ?
take your food sample and
grind
this with
distilled water
using a
motar
and
pestle
(make a paste)
Transfer the paste to a
beaker
and add
distilled water.Stir
so the chemicals in the food
dissolve
in the
water
Filter
the solution and remove the
suspended particles
How do you
test
for starch ?
place
2cm
^
3
of food solution to a
test tube
add a few drops of
iodine
solution (its orange )
if the solutions
blue-black
it means starch is present
if it stays orange it means there is no starch
How do you test for sugar ?
place
2cm
^
3
of food solution into a test tube
add
10
drops of
benedicts
solution solution (its
blue
)
place the test tube into a a beaker and fill it half way with
hot water
from a
kettle
(leave it for
5
minutes )
if sugars present the benedicts solution will change
colour
What does the colour of benedicts solution tell us ?
gives us an
approximate idea
of how much
sugar
there is
What does a green colour of benedicts solution tell us ?
there is small amount of sugar
What does a yellow colour of benedicts solution tell us ?
there is
more sugar
present
What does a brick-reddish colour of benedicts solution tell us ?
there is
alot
of
sugar
present
The
Benedicts
test only works for certain sugars e.g
glucose
How to test for protein ?
place
2cm^3
of food solution
add
2cm^3
of biuret solution (its
blue
)
if the solution turns
purple
/
lilac
colour protein is present
How to test for lipids ?
place
2cm
^
3
of food solution (which is not
filtered
)
add a few drops of
distilled water
and
ethenol
gently
shake
the solution
if lipids are present then
white cloudy emulsion
will form
Why do you not filter the food solution for lipid ?
lipid molecules
will
stick
to the
filter paper