lab quiz exp 1-5

• increasing magnification enlarges a specimen. increasing resolution provides better detail.
• resolution is the ability of a viewing instrument to distinguish adjacent objects as separate rather than as one larger object
• the lens of the human eye has the resolving power of 100 um, meaning if the space is 90 um, an object will appear as a single object
• the best light microscopes have a resolving power of 1 nm
• empty magnification is when a larger image has no greater detail
• when ultra-fine structures of an enlarged image is presented, this is called useful magnification
• the quality of lens system and wavelength of source of illumination are the two most important factors that determine the resolving power
• resolution varies inversely with wavelength, as the shorter the wavelength, the better resolution.
• the eyepiece lens are the lens at the top that you look through
• eyepiece lens are usually 10x-15x in power
• the tube is what connects the eyepiece to the objective lenses
• the arm supports the tube and connects it to the base
• the illuminator is a steady light source (110 volts) used in place of a mirror
• A mechanical stage has two knobs (L-R, U-D)
• rack stop is an adjustment that determines how close the objective lens can get to the slide
• rack stop keeps students from cranking HPO lens into slide and break them
• Condenser lens focuses light onto specimen. They are most useful at high powers.