Biuret test

• what test is used to test for proteins?

  Biuret test
• how many stages in the biuret test?

  two
• what is the 1st stage of the biuret test?

  The test solution needs to be alkaline, so first add a few drops of sodium hydroxide solution
• what is the SECOND stage of the biuret test?

  add some copper(II) sulfate solution
• what happens if protein is present?

  solution turns purple
• what happens if protein IS NOT present?

  solution stays blue

  • the colours are pale so look carefully