LESSON 2

• Types of Glass

  • Borosilicate Glass
  • Boron-Free Glassware
  • Corex
  • Vycor
  • Flint Glass
  • Plastic Wares
• Borosilicate Glass


  • Pyrex and Kimax - brands
  • Most commonly used glassware
  • Used for heating and sterilization purposes
  • Characterized by a high degree of thermal resistance and low alkali content
• Boron-Free Glassware


  • Soft glass
  • High resistance to alkali
  • Its thermal resistance is less compared to borosilicate glass
• Corex


  • Corning
  • Special alumina-silicate glass that has been strengthened chemically than thermally
• Vycor


  • Corning
  • Utilized for high thermal, drastic heat
  • Characterized by its very low coefficient of expansion and correspondingly high resistance to heat shock, its high softening point, low power factor, and high chemical durability, which make it useful for varied applications in the industrial and electrical fields as well as in the laboratory
• Flint Glass


  • Made up of soda-lime glass and a mixture of Calcium, Silicon, and Sodium Oxides
  • Poor resistance to high temperature
• Plastic Wares

  • Polyolefins
  • Fluorocarbons
  • Engineering Resins
  • Labware Plastics
• Polyolefins

  • Polyethylene
  • Polypropylene
• Fluorocarbons

  • Teflon
  • PDUF
• Engineering Resins

  • Nylon
  • Acetol
  • Polycarbonate
  • Polystyrene
  • Polyphenylene Oxides
• Labware Plastics

  • Corian
  • Epoxy resins
  • ABS
  • Polyetherimide
• Calibration

  1. Marks/Design

  2. Drainage Characteristics
• Types of Pipette

  • Transfer
  • Graduated
  • Measuring
• Calibration Marks/Design

  1. TO DELIVER (TD)

  2. TO CONTAIN (TC)
• Drainage Characteristics

  1. BLOWOUT

  2. SELF-DRAINING
• Transfer Pipette

  • Volumetric Pipette
  • Ostwald Folin
  • Pasteur Pipette Automatic Micro or Macro Pipettes
• Drainage Characteristics

  • Blown out
  • Self-draining
• Graduated or Measuring Pipette

  • Serological, Mohr, Bacteriologic, Kolmer and Kahn, Micropipettes
• Transfer Pipette

  • Self-draining
  • For non-viscous fluid
• Volumetric Pipette

  • Self-draining
  • For non-viscous fluid
• Ostwald Folin

  • For viscous fluid
  • With etched ring
  • Bulb is lower than the volumetric pipette
• Pasteur Pipette

  • Commonly made from plastic
  • Transfers fluid without consideration of specific volume
• Serological Pipette

  • With graduations to the tip
  • Blowout pipette
• Mohr Pipette

  • Without graduations to the tip
  • Self-draining pipette
• Micropipettes

  • Sahli-Hellige Pipette
  • Lang-Levy Pipette
  • RBC and WBC Pipette
  • Kik and Overflow Pipette
• How to use a Pipette

  1. Keeping your finger on the end of the pipette, gently move it to the waste container

  2. Touch the tip to the inside of the container, lift your finger off the end and allow the liquid to drain out of the pipette

  3. Hold the pipette in this position for a few seconds after it stops draining
• Automatic Pipettes

  • Micropipettes
  • Macropipette
• Classification according to mechanism

  • Air-displacement
  • Positive displacement
  • Dispenser/Dilutor