VENIPUNCTURE (During Lab Discussion)

• Venipuncture has similar set/procedures on dermal puncture, the only difference is the materials
• Phlebotomy procedure


  1. Request form serves as test request

  2. Disinfect the area by spraying alcohol and wipe it using rag or tissue

  3. Introduce yourself to the patient

  4. Explain the procedure

  5. Ask for latex sensitivity/allergy

  6. Ask if the patient fasts and about their last meal

  7. Identify the patient

  8. Start with vein selection

  9. Position the patient

  10. Palpate the vein

  11. Remove tourniquet

  12. Disinfect the site

  13. Prepare materials while air drying the site

  14. Lock and inspect the needle

  15. Re-apply the tourniquet

  16. Anchor the vein

  17. Insert the syringe

  18. Collect blood

  19. Release the tourniquet

  20. Instruct patient to apply pressure on the site

  21. Fish out the cap of the needle

  22. Dispose needle in sharps container

  23. Open tubes and transfer blood

  24. Inversion of tubes

  25. Label the tube

  26. Stick tube stickers on the request form

  27. Inspect the site after procedure

  28. Thank the patient and clean the area
• No gloves are allowed in palpating the vein, but gloves are needed during the collection proper
• If blood appears on the hub once you hit the vein

  Then pull the plunger to collect
• If the plunger resists when pulling, it means you're out of the vein