W5L10 - Nucleic acid hybridisation MCQ

• Nucleic acid hybridisation is when...

  1. None of the above.
  2. double-stranded DNA or RNA molecules anneal to complementary DNA or RNA.
  3. double-stranded DNA or RNA molecules anneal to non-complementary DNA or RNA.
  4. single-stranded DNA or RNA molecules anneal to complementary DNA or RNA.
  5. single-stranded DNA or RNA molecules anneal to non-complementary DNA or RNA.

  4
• Which of the following will contribute to the melting temperature of DNA?

  1, 3, 5, 7, 10

  
• Which of the following techniques use DNA hybridisation?

  1. Chromosome in situ hybridisation
  2. Colony blotting
  3. Dot blotting
  4. ELISA
  5. Gene editing (CRISPR/Cas9 system)
  6. Macroarrays
  7. Microarrays
  8. Northern blotting
  9. PCR
  10. Southern blotting
  11. Western Blotting

  1, 2, 3, 5, 7, 8, 9, 10
• Which enzyme is used in PCR?

  1. Ligase
  2. RNA polymerase
  3. Taq polymerase
  4. Top polymerase
  5. Topoisomerase

  3
• Which of the following are true when comparing Northern and Southern blotting?

  1. Northern and Southern blotting both have DNA on the filter membrane.
  2. Northern and Southern blotting both have RNA on the filter membrane.
  3. Northern and Southern blotting can only use fluorescent probes.
  4. Northern blotting has DNA on the filter membrane; Southern blotting has RNA.
  5. Northern blotting has RNA on the filter membrane, Southern blotting has DNA.
  6. The probes used in Northern and Southern blotting can be fluorescent or radio-labelled.

  5 and 6
• Which of the following are true about hybridisation stringency?

  1. Decreasing the temperature decreases stringency.
  2. Decreasing the temperature increases stringency.
  3. Increasing NaCl concentration decreases stringency.
  4. Increasing NaCl concentration increases stringency.
  5. Increasing the temperature decreases stringency.
  6. Increasing the temperature increases stringency.

  1, 3, 6
• Microarrays can be used...

  1. for mutation screening.
  2. identifying single nucleotide polymorphisms (SNPs).
  3. to determine the levels of protein in a cell.
  4. to examine the levels of gene expression.
  5. to mutate a particular sequence.


  1, 2, 4
• CRISPR requires which of the following:

  1. Cas10
  2. Cas8
  3. Cas9
  4. Double-stranded DNA target
  5. PAM sequence
  6. POM sequence
  7. guide DNA
  8. guide RNA

  3, 4, 5, 8
• Which is the correct order of steps in Northern blotting?

  • Develop the result
  • Transfer DNA to a filter membrane 
  • Restriction digest
  • Run sample on a gel
  • Add the hybridisation probe

  Restriction digest - run sample on a gel - transfer DNA to a filter membrane - add the hybridisation probe - develop the result