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Topic 1 key concept biology
BIOLOGY Core practical
Core practical:investigate effects of ph on enzyme activity
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Cards (13)
Step 1:
Set up water bath
at
35°
Step 2:
put drop
of
iodine solution
in
each dimple
of
spotting tile
Step 3:measure
5cm
^
3
of
starch solution
and
2cm
^
3
of
buffer solution
(
record ph
) into
2 test tubes
Place test tues in water bath and wait for them to reach same temperature as
water bath
Step 4:
add 5cm
^
3
of
amylase solution
to
one
of the
test tubes
and
2cm
^
3
of
distilled water
to
other
What does the test tue with distilled water act as
Control
Step: start stopwatch and after 30seconds use 2 pipettes to transfer a few drop of each test tube mixture on spotting tile
Step6: obserse if
iodine
on
spotting tile
turns
blue-black If mixture with amalyse turns
iodine blue black the
analysis
has
starch
to
break down
What will control mixture always turn iodine
Blue-black
Step 7:
repeat steps 4-6 every 30 seconds until mixture with amylase
stops
turning iodine blue-black
Step 8:
record time it took for iodine to stop turning
blue-black in
results table
Step9:
repeat method
for
remaining buffer solutions
The
shorter
the time the
faster amylase
has worked to
break down starch
