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Biology
Microscopes
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Resolution :
Smallest distance
between
two points
that can still be seen as
two points
, in other words, as
separate identities
If an image has a
high resolution
, it means that it has a
higher amount
if
pixels
Magnification
(to
magnify
) : How many time you have
increased
the
image
from the
actual specimen
Equation of
magnification
->
Magnification
=
Size
of the
image
/
size
of the
actual object
The
light microscopes
were invented around
1590
The
light microscopes
allow us to see the fine details of an object , like
cells
and sometimes
bigger organelles
The
electron microscope
e invented in
1931
The electron microscope use beans of
electrons
while the
light microscope
uses rays/beans of
light
The
electron microscope
has a
large depth
of
field
, it allows
more specimen
to be focused at
one time
The electron microscope has a much
higher resolution
and
magnification
While using a light microscope you should always use the
smallest lens
first
Field of view
: How much you're able to see of a magnified sample at a specific level and is displayed in distance unit
While using a microscope you should add
water
(
like
a
drop
) to the specimen for it to
expand
While using a
microscope
you may want to add a
stain
to the
specimzen
on the
slide
so that it "
expand
" the
particles
and make it
easier
to
see
While using a
microscope
, you should press down on the
cover slip gently
so as not to
smash
the
sample
too much
Eyepiece lens
: It is a lens that is closest to your eye .It usually have a
magnification
of
x10
Objective lens : There are
three lens
of
different
strenghts which can be used to
magnify
the image more
clearly
Stage
: This is the
pplace
where you put a
microscope slide
.
Clips
hold the
slide
in place
Diaphragm
: This holds the
eyepiece lens
above the
stage
Light
:
Control
the
amount
of
light
that goes onoto the
microscope slide
Base
: The
light source
projects
light
onto the
microscope slide.It
can be a
mirror
or an
electric bulb
Arm
: This is the very
heavy
part that keeps the
microscope
into place from falling over
Fine focus
knob : This is used to focus the image so its very sharp and clear
Coarse focus
knob : This is used to focus the image so that you cans ee it clearly
the
light microscope
was invented by
Antoni van Leeuwenhoek