Advantages of In Vivo cloning

• One advantage of in vivo cloning is that it is useful when introducing a gene into another organism. Since in vivo cloning involves the use of vectors, once we have introduced the desired gene into a plasmid, the plasmid can be used to deliver the gene into another organism during gene therapy.
• Another advantage of in vivo cloning is that there is almost no risk of contamination, since the gene has been cut by the same restriction endonuclease used to cut the vector. Therefore, contaminant DNA won't have complementary sticky ends, so won't be taken up by the plasmid.
• Another advantage of in vivo cloning is that it is very accurate, with minimal errors. Also, this technique involves cutting out specific genes, making it a very precise procedure as the culturing of the transformed bacteria produces many copies of a specific gene, not just copies of the entire DNA sample.
• One disadvantage of in vivo cloning is that it is very time consuming and requires monitoring of cell growth.