RBC AND PLT PRESERVATION

avatar
Created by
Alyssa Marie

Cards (56)

  • CITRATE BINDS IONIZED CALCIUM
  • ADENINE INCREASE ADP LEVEL
  • GLUCOSE SERVE AS FOOD FOR THE CELLS
  • PHOSPHATE PROMOTES OXYGEN RELEASE TO TISSUES
  • HEPARIN: 2 DAYS
  • 21 DAYS: ACD (ACID-CITRATE-DEXTTROSE), CP2D( CITRATE-PHOSPHATE-DOUBLE DEXTROSE), CPD (CITRATE-PHOSPHATE-DEXTROSE)
  • 35 DAYS: CPDA-1 (CITRATE-PHOSPHATE-DEXTROSE ADENINE)
  • ADDITIVE SOLUTIONS - SOL. ADDED TO RBC AFTER REMOVAL OF PLASMA W/ OR W/OUT PLATELET (SALINE, ADENINE , MANNITOL, GLUCOSE). 45 DAYS OF STORAGE.
  • REJUVINATING SOLUTIONS USED TO RESTORE ATP AND 2,3DPG LEVELS. (PHOSPHATE,INOSINE,GLUCOSE, PYUVATE AND ADENINE. RBCS STORED IN LIQUID STATE AFTER REJUVINATE 3 DAYS PRESERVATION.
  • Atleast 70% of the red blood cells must remain viable at the end after storage period.
  • 75% of blood transfused should remain viable after 24 hrs of transfusion.
  • Shipment of blood is maintained at temperature of 1-10 degree celcius. Ice or other cooling devices should not physically be in contact with the blood unit to prevent hemolysis.
  • Storage Lesions biochemical changes in stored blood that can be lead to decreased RBC viability.
  • Packed RBCs - can be prepared through centrifugation
  • Heavy spin ( 5000 x g for 5 minutes) when temperature is set at 4 degree celcius and if the whole blood used is not intended for platelet concentrate preparation
  • Light spin (3200 x g for 2-3 minutes; or 2000x g for 3 minutes) when temperature is at 22-degree celcius and if the whole blood is to be used for platelet concentrate preparation.
  • Platelet Concentrate -can be prepared FROM WB centrifugation.
  • platelet derived from WB as RDP(random donor platelet)
  • whole blood to be used for preparation of RDP must be processed within 6-8 hours after collection( 6 hours if ACD; 8 hours for CPD,CP2D,CPDA1)
  • whole blood to be used for RDP preparation must be maintained at 20-24C.
  • unit should contain t least 5.5x10 platelets
  • unit must be contain sufficient plasma to maintain a pH greater than or equal to 6.2
  • platelets are stored at 20-24C for 5 days in constant agitation.
  • allow the platelet concentrate to remain undisturbed for 1-2 hours at 20-24 C. This is to enhance platelet disaggregation. Resuspend by gentle manipulation.
  • platelet unit obtained from plateletpheresis is known as SDP(Single donor platelet)
  • HLA(human leukocyte antigen) alloimmunization is a result of a multiple platelet transfusion resulted to ineffective platelet transfusion this is due to the develop of platelet antibodies and HLA antigen.Thus it is best to transfuse platelet units collected via plateletpheresis.
  • Fresh Frozen Plasma (FFP)- plasma has been prepared w/in 6 hours after collection (if ACD is used), 8 hours after collection if (CP2D,CPD, CPDA1 is used).
    • Plasma frozen within 24 hrs (PF24)- if platelet has been prepared within 8-24 hours after collection.
  • initially stored horizontal but if frozen, shifted to vertical position.
  • stored at -18C for 1 year or at -65 for 7 years
  • must be thawed before transfusion @ 30-37C with agitation in water bath.
  • once the product is completely thawed, store the product for about 1-6C and should be transfused within 24 hrs.
  • . Cryoprecipitate prepared from FFP allow the FFP to thaw in a refrigerator @ 1-6 C for about 14-16 hrs.
  • a precipitate is recovered after a hard spin from plasma called cryoprecipitate.
  • supernatant fluid can be frozen at -18 C and labeled as plasma cryoprecipitate reduced also known as cryosupernate.
  • whole blood modified can be modified by having 50 ml of plasma removed in preparation of platelets, or 10-15 mL of plasma removed in the preparation of cryoprecipitate.
  • irradiated components is performed in order to inactivate the lymphocytes, which can cause graft- vs host disease (gvHD) it is done to a immunocompremised/ immunosuppressed and donations to immunocompetent first degree of relatives commonly used irradiating blood components is the comercially availbale CESIUM source
  • Leuko-poor or Leuko-reduced components RBC units and platelet units can undergo leuko-reduction in order to reduce the number of leukocytes.
  • pre-storage leuko-reduction - WBCs are removed prior to storage. It can help to prevent febrile transfusion reactions caused by both WBC and BRMs(biologic response modifiers).
  • post-storage leuko-reduction- since it undergone storage, there is an accumulation already of BRMs. Thus only the WBCs being removed not the BRMs.