Add the iodine solution directly to the substance to be tested and look for a colour change. If it stays brown it is negative and if it changes to black it is positive.
Method of testing sugar?
Add Benedict's reagent to the solution to be tested. Heat for two minutes in a water bath at boiling point and look for a colour change. If it stays blue there is no sugar, and if it changes to red it contains sugar, but if it turns green/yellow/orange it has less sugar.
Method of testing fats?
Add ethanol to the solution to be tested and shake thoroughly. Then add water and look for a colour change. If it stays clear it has no fats and if it turns cloudy white it has fats.
Method of testing protein?
Add Biuret solution to the solution to be tested and look for a colour change. If it stays pale blue it doesn't contain protein, if it changes to purple it contains protein.
If the iodine stays brown it has no starch, if it changes to black it contains starch.
If the Benedict's reagent stays blue it doesn't contain sugar, if it turns red it contains sugar, if it turns green/yellow/orange it has less sugar.
If the ethanol stays clear it doesn't contain fat, if it changes to cloudy white it contains fats.
If the Biuret solution stays blue it doesn't contain protein, if it changes to purple it contains protein.