Stewart Linn and Werner Arber discovered restriction enzymes in E. coli, which are known as endonucleases
In 1969, Herbert Boyer isolated the restriction enzyme EcoRI from E. coli. That cleaves the DNA between G and A in the base sequence GAATT.
In 1970, Howard Temin and Davin Baltimore independently discovered the enzyme reverse transcriptase from retroviruses. This enzyme was used to construct a DNA called complementary DNA (cDNA) from any mRNA
In, 1972 David Jackson, Robert Symons and Paul Berg successfully generated rDNA molecules.
In 1973 for the first time S.Cohen and H.Boyer developed a recombinant plasmid (pSC101) which after using as vector replicated well within a bacterial host.
In, 1975, Edwin Southern developed a method for detection of specific DNA fragments for isolation of a gene from complex mixture of DNA
Luther Burbank created a disease resistant potato called the Burbank potato. He crossed a disease resistant plant with one that had a large food producing capacity
In 1749, George Louis Leclerc, known as Count Buffon, a French naturalist, first described descent with modification such as environmental influences, migration, geographical isolation and the struggle for existence. However he was hesitant in sharing his idea to the public.
In 1795, Georges Cuvier, a vertebrate zoologist and paleontologist proposed the theory of catastrophism.
In 1830, in contrast to catastrophism, James Hutton and Charles Lyell believed that natural forces now changing the shape of the earth’s surface have been operating in the past much the same way.
Jean Baptiste de Lamarck was the first biologist to believe that life forms evolve. He proposed the Inheritance of Acquired Characteristics, which is also called Lamarckian Evolution.
In 1859, Charles Darwin published his book, On the Origin of Species by Means of Natural Selection.
Charles Darwin however, was not the first to publish his theories involving natural selection. In 1858, Alfred Russel Wallace a British naturalist working in the South Pacific islands of the Malay Archipelago, had submitted his ideas for publication.