Restriction Enzyme

• Restriction enzyme


  A protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end
• Recognition site


  The DNA sequence where the restriction enzyme will cut
• Enzyme name


  Depends on the organism the enzyme is isolated from
• The cut DNA from two-parent DNAs must be compatible and follow the Chargaff Rule
• The sticky ends are not literally sticking together, the ligase will glue the invisible gaps