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Chapter 2: Specimen Collection and Processing
STOOL FOR OVA AND PARASITE EXAMINATION
Fixatives for Preservation
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Thatus Licudan
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Cards (10)
A fixative that is commonly used for preserving protozoa and helminths but does not adequately preserve parasite morphology for permanent smears?
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Formalin
A fixative that is a combination of polyvinyl alcohol and Schaudinn solution, suitable for preparing permanent stained smears?
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Polyvinyl alcohol (PVA)
A fixative is mercury-free and suitable for concentration techniques, permanent stained smears, and detecting coccidian oocysts?
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Sodium acetate formalin (SAF)
A fixative is an alternative to mercury-based PVA and can be used for concentration methods and permanent stained smears?
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Modified polyvinyl alcohol fixative
A fixative that is free of formalin and mercury and can be used for concentration techniques, permanent stained smears, and fecal immunoassays?
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Alternative single-vial systems
What is the recommended ratio of fixative to stool when preserving a stool sample?
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3 parts fixative to 1 part stool or 1:3
Which fixative has a long shelf life and can preserve specimens for several years?
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Formalin
It is fixative that requires the addition of albumin to ensure specimen adhesion on slides?
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Sodium acetate formalin (SAF)
A fixative that contains mercuric chloride and has led to regulations and a search for alternatives?
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Polyvinyl alcohol (PVA)
A fixative that may reduce the clarity of protozoa morphology compared to mercury-based fixatives?
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Sodium acetate formalin (SAF)