Direct Wet Preparation

• What is the primary purpose of a direct wet preparation?

  = Detecting motile protozoan trophozoites
• Fresh specimens of which consistency are preferred for direct wet preparations?

  = Liquid
• If a stool specimen is received in a fixative, what can be done regarding the direct wet preparation?

  =  Skip the procedure entirely
• Which procedure is recommended as a higher priority over the direct wet preparation?

  =  Concentration procedure
• In a direct saline wet preparation, what is mixed with the stool specimen on a glass slide?

  =  Saline
• How should the slide be examined when performing a direct wet preparation?

  = Starting with the low-power objective (10×)
• When is oil immersion usually recommended for direct wet preparations?

  = When using a high-power objective (100×)
• What is the purpose of using iodine in a direct wet preparation?

  = To enhance the detail of protozoan cysts
• Can both saline and iodine wet preparations be prepared simultaneously?

  = Yes, they can be prepared side by side on a large slide
• How much time do skilled laboratory technicians typically spend screening a slide with proper adjustments?

  = Around 10 minutes