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Chapter 2: Specimen Collection and Processing
OTHER SPECIMENS AND LABORATORY TECHNIQUES
Eye Specimens
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Thatus Licudan
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Cards (10)
What is the recommended method for diagnosing Acanthamoeba keratitis?
=
Corneal scrapings
How should corneal scrapings for Acanthamoeba keratitis be collected?
= In an
airtight
container
What can be tested in addition to corneal scrapings for Acanthamoeba keratitis?
=
Contact lens
or
contact lens solution
Which method can be used to culture Acanthamoeba from corneal scrapings?
Culture on blood agar plate
Molecular testing using PCR
Culture on Sabouraud agar plate
Culture on agar plate seeded with gram-negative bacteria
=
Culture on agar plate seeded with gram-negative bacteria
How can Acanthamoeba cysts be visualized using microscopic examination of corneal scrapings?
=
Calcofluor white stain
Which color do Acanthamoeba cysts stain with the calcofluor white stain?
=
Green
Which eye pathogen can be detected using histologic stains and specialized culture methods?
Toxoplasma gondii
Trypanosoma cruzi
Giardia lamblia
Plasmodium falciparum
=
Toxoplasma gondii
What is the potential eye pathogen associated with microsporidia infections?
=
Microsporidia itself
Which microscopic examination technique is used to visualize Acanthamoeba cysts?
Fluorescent microscopy
Electron microscopy
Phase-contrast microscopy
Bright-field microscopy
=
Fluorescent microscopy
How can Loa loa infection be detected in eye specimens?
=
Histologic stains
and
specialized culture methods