Skin Snips and Culture Methods

• Skin snips are useful in the detection of what parasite?

  = Onchocerca volvulus
• What is the objective of skin snip procedures?

  =  To obtain skin fluid without bleeding
• How many collection techniques are typically used for skin snips?

  = Two
• What tool is used in one of the skin snip collection techniques?

  = Scleral punch
• What is the purpose of making a small cut into the skin in the other skin snip collection technique?

  = To obtain skin fluid
• What should be done with the material obtained from skin snips?

  = It should be placed in 0.2 mL of saline.
• How long is the incubation period for the material obtained from skin snips?

  = 30 minutes
• What is the purpose of the incubation period for the material obtained from skin snips?

  = To induce motility in the microfilariae
• How can the presence of microfilariae be detected in the saline during microscopic examination?

  = They exhibit jerky movements.
• Where do the microfilariae tend to migrate into the liquid from during the incubation period?

  =  Skin snip itself
• Which of the following parasites can be isolated using culture methods?

  • Enterobius vermicularis
  • Toxocara canis
  • Giardia lamblia
  • Plasmodium falciparum

  = Giardia lamblia
• Which culture method is commonly used for the isolation of Giardia lamblia?

  • Sabouraud agar culture
  • MacConkey agar culture
  • Jones' medium culture
  • Blood culture

  = Jones mediem culture
• Which of the following parasites cannot be isolated using culture methods?

  • Trichinella spiralis
  • Toxoplasma gondii
  • Trypanosoma brucei
  • Cryptosporidium parvum

  = Cryptosporidium parvum
• What is the primary purpose of culture methods in parasitology?

  = To isolate and grow parasites in the laboratory
• Which of the following parasites can be cultured using axenic culture methods?

  • Schistosoma mansoni
  • Trichomonas vaginalis
  • Trypanosoma cruzi
  • Plasmodium vivax

  = Trichomonas vaginalis
• Which specialized culture medium is commonly used for the isolation of Entamoeba histolytica?

  • Löwenstein-Jensen medium
  • Sabouraud agar
  • Robinson's medium
  • Lowenstein-Jensen medium

  = Robinson's medium
• Which of the following techniques is used for the culture of Leishmania spp.?

  • Blood culture
  • Axenic culture
  • Enrichment culture
  • Tissue culture

  = Axenic culture
• Which parasite can be isolated using xenic culture methods?

  • Plasmodium malariae
  • Naegleria fowleri
  • Toxocara cati
  • Trichuris trichiura

  = Naegleria fowleri
• Which of the following is a commonly used culture method for Trypanosoma cruzi?

  • Blood culture
  • Sabouraud agar culture
  • Chocolate agar culture
  • MacConkey agar culture

  = Blood culture
• Which parasite is typically cultured using the McCoy cell line?

  • Strongyloides stercoralis
  • Toxoplasma gondii
  • Wuchereria bancrofti
  • Taenia solium

  = Toxoplasma gondii