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Module 2
2.3:NUCLEOTIDES+NUCLEIC ACIDS
DNA precipitation
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Aaron
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Cards (5)
homogenise cell with
detergent
to break open the
cell membrane
which helps
release
its contents
2)
filter
out to remove large
debris
3) add
salt
to break
hydrogen bonds
between
DNA
and
water
4) add
protese
enzyme to digest the
proteins
5) add
ice cold ethanol
do
precipitate
out the
DNA
from solution . DNA will appear as
white
strands