Laboratory Diagnosis

• Which of the following is NOT a standard method used for the laboratory diagnosis of E. histolytica infection?A)Wet preparation of stool samplesB) Permanent staining techniques on stool samplesC) Examination of material collected from sigmoidoscopyD) Culture on TYI-S-33 medium

  = Culture on TYI-S-33 medium
• In addition to stool samples, which other materials can be examined using standard methods for E. histolytica diagnosis?

  = Material collected from sigmoidoscopy or hepatic abscess
• Which medium supports the growth of E. histolytica in culture?

  • Sabouraud agar
  • MacConkey agar
  • Lowenstein-Jensen medium
  • TYI-S-33 medium

  = TYI-S-33 medium
• Which of the following is NOT an immunologically based procedure used for the diagnosis of E. histolytica?

  • Enzyme-linked immunosorbent assay (ELISA)
  • Indirect hemagglutination (IHA)
  • Polymerase chain reaction (PCR)
  • Antigen tests

  = Polymerase chain reaction (PCR)
• Serologic tests for E. histolytica are typically more helpful in cases of:

  =  Extraintestinal infections
• What test is based on the detection of specific E. histolytica antigens?

  =  ELISA
• What method involves the use of antibodies to detect E. histolytica in patient samples?

  = ELISA
• The examination of material collected from a sigmoidoscopy procedure is particularly useful for diagnosing:

  = E. histolytica infection
• Which of the following is NOT an alternative method used for the laboratory diagnosis of E. histolytica?

  • Antigen tests
  • ELISA
  • Wet preparation of stool samples
  • Culture on TYI-S-33 medium

  = Wet preparation of stool samples