Laboratory Diagnosis

• Which method is considered the method of choice for the recovery of N. fowleri ameboid trophozoites?

  Microscopic examination of CSF
• What type of preparations should be prepared and scanned for the microscopic examination of CSF?

  Saline and iodine wet preparations
• In addition to CSF, which other specimens may be examined for the diagnosis of N. fowleri?

  Tissue samples and nasal discharge
• What is the recommended method for culturing N. fowleri from clinical specimens?

  Agar plates previously inoculated with gram-negative bacilli
• How do N. fowleri ameboid trophozoites react when placed on agar plates previously inoculated with gram-negative bacilli?


  Exhibits a characteristic trailing effect
• What is the primary advantage of microscopic examination of CSF in the diagnosis of N. fowleri?

   High sensitivity
• Which staining method is commonly used for the microscopic examination of N. fowleri?

   Giemsa stain
• Which of the following diagnostic methods is not used for N. fowleri detection?

  Imaging studies
• What is the key advantage of culturing clinical specimens for N. fowleri?

  High specificity
• Which of the following is the gold standard method for identifying N. fowleri?

  Culture