Notes of Interest and New Trends

• Acanthamoeba shares characteristics with which gram-negative bacteria?

  Pseudomonas aeruginosa
• True or False: Acanthamoeba and Pseudomonas aeruginosa are frequently recovered simultaneously from the same patient.

  False
• What is believed to be the effect of Pseudomonas aeruginosa on Acanthamoeba?

  Inhibition of activity
• In a rare case, cutaneous lesions filled with Acanthamoeba were observed in a patient with AIDS. In addition to the skin lesions, what other condition did the patient present with?

  Brain lesion
• Acanthamoeba invasion of bone was observed in a patient following a graft procedure. What complication did the patient subsequently develop?

  Osteomyelitis
• Which testing method aims to differentiate strains of Acanthamoeba?

  Monoclonal antibodies
• What is the purpose of DNA RFLP tests in the classification of Acanthamoeba?

  Taxonomic classification
• True or False: Additional testing with newer techniques for Acanthamoeba differentiation and classification has not shown promising results.

  False
• Which of the following is NOT a newer testing method discussed for Acanthamoeba differentiation?a)Flow cytometryb) Monoclonal antibodiesc) DNA RFLP testsd) Gram staining

  Gram staining
• What is the suggested approach regarding additional testing with newer techniques for Acanthamoeba differentiation?

  Conduct further research before implementing them