Principles of tissue culture
1. Removal of meristematic cells from the plant (explant)
2. Explant is immersed in a well-aerated solution containing a balance of auxin and cytokinin to stimulate cell growth and division and nutrients (sucrose, and inorganic ions K, Mg and nitrates) and is sterile to prevent fungi and bacteria
3. Undifferentiated cells in the explants divide by mitosis to produce callus
4. A callus is placed in agar jelly with nutrients to produce root and shoot
5. When the plant is large enough it's placed in sterile soil