Microbiology practical

• Microbiology practical

  1. Disinfect working area and hands
  2. On the bottom of agar plate draw 3 segments
  3. Place different antiseptics onto different filter paper discs
  4. Using forceps place each filter paper disc into a each different segment
  5. Tape the lid
  6. Place agar plate into incubator for 48 hours
  7. Measure the diameter of clear zones
  8. Take second and third measurement to find the mean
  9. Calculate the area using πr2
• Aseptic technique method;

  1. Pre-sterilised petri dishes can be used, so there is no bacteria.
  2. Pour the sterile agar plates, provides nutrients needed to grow.
  3. Sterilise the inoculating loop, by heating it with a Bunsen burner, this kills all bacteria.
  4. Use inoculation loop to make streaks on agar with bacteria, allowing it to spread out.
  5. Put on lid, secure with tape, and store upside down. This prevents airborne bacteria for contaminating it and stops harmful anaerobic bacteria from growing.
  6. Incubate at 25°C in schools and colleges. This ensures that bacteria can't survive in human body.