Enzymes practical

• Safety precautions for enzymes practical:

  -Careful using hot water

  -If using bunsen burner, tie hair and use goggles
• Sources of error for enzymes practical:

  -Intervals between testing may be too long to accurately find time taken for starch to completely breakdown
• Enzyme practical : - I part 1

  1. Set up a Bunsen burner, heatproof mat, tripod and gauze.
  2. Place a beaker of water on the gauze and adjust the flame to keep the water at about 35°C.
  3. Now put two drops of iodine solution into each spot of a spotting tile.
  4. Add 2 cm3 of amylase enzyme solution to a test tube.
  5. Place 2 cm3 of starch solution into the same tube.
  6. Finally add 1 cm3 of pH solution to the tube. This will keep the pH constant.
• Enzyme practical: - part 2

  7. Mix the solution in the test tube and place it into the beaker of water on the Bunsen burner. 8. Use a pipette to remove a few drops of solution every 20 seconds from the test tube and put them into a different well of the spotting tile.

  9. Repeat until the iodine solution stops turning black.

  10. Record the time this takes.

  11. Repeat with different pH solutions