Enzymes practical

• Safety precautions for enzymes practical:

  -Careful using hot water

  -If using bunsen burner, tie hair and use goggles
• Sources of error for enzymes practical:

  -Intervals between testing may be too long to accurately find time taken for starch to completely breakdown
• Enzyme practical method:

  1. Place a drop of iodine solution into each spot on the spotting tile.
  2. Into one test tube add starch solution. And in the other add 1 cm^3 of buffer solution and 2 cm^3 of amylase solution.
  3. Place the test tubes into a warm water bath.
  4. Then quickly pour amylase and buffer solution into test tube with starch and start stopwatch.
  5. Using a pipette take some mixture add place into spotting tile.
  6. Wait 10 seconds and take another drop from mixture and add to spotting tile.
  7. Repeat every 10 seconds until iodine doesn't turn black anymore and stays orange.
  8. Repeat experiment with different pH values.