Male damselflies transfer sperm during an unusual mating flight, where the male and female genitalia of each species are uniquely shaped and physically incompatible with other species
Male gametes may not be able to recognize and fertilize an egg of a different species, such as in many marine animals where sperm recognizes eggs of their own species through chemical markers
Mating and fertilization are possible, but genetic differences result in a zygote that is unable to develop properly, such as in some species of sheep and goat