CARBS

• Glycolysis decreases serum glucose by approximately 5-7% in 1 hour
• Alkaline Copper Reduction Method is thereduction of cupric ions to cuprous ions forming cuprous oxide in hot alkaline solution by glucose.
• Alkaline Copper Tartrate + Cuprous Ions = Alkaline Copper Reduction Method
• Benedict’s Method is used for detection and quantitation of reducing substances in body fluids like blood and urine.
• Benedict’s Method uses citrate as stabilizing agent
• Alkaline Ferric Reduction Method involves reduction of a yellow ferricyanide to a colorless ferrocyanide by glucose (Inverse Colorimetry)
• Hagedorn Jensen another term for alkaline ferric reduction method
• Ortho-toluidine is the condensation of glucose with a primary aromatic amine in glacial acetic acid, forming an equilibrium mixture of a glycosylamine and the corresponding Schiff base
• –Glucose in a PFF (3% TCA) reacts to O-Toluidine in hot acidic solution will yield a GREEN colored compound with maximum absorbance at 630 nm
• Increased levels of uric acid, bilirubin, and ascorbic acid can cause falsely decreased values.
• Polarographic Glucose Oxidase measures rate of oxygen consumption which is proportional to glucose concentration
• MOST SPECIFIC GLUCOSE METHOD; REFERENCE METHOD is the hexokinase method
• glucose dehydrogenase is when Glucose is reduced to produce a chromophore that is measured spectrophotometrically or an electrical current
• DEXTROSTICS is An enzyme-impregnated strip used with a small portable electronic colour-measuring device for convenient estimation of the blood sugar levels by diabetics.
• INTERSTITIAL GLUCOSE MEASURING DEVICE uses electrochemical methods to automatically and frequently measure glucose levels in the interstitial fluid of dermis or subcutaneous fat tissue.
• Random plasma glucose is a glucose testing where specimens collected anytime and usually done in a glucometer
• Fasting plasma glucose is hwen specimen are collected ftaer 8-10 hours of fasting
• fructosamine is wideley used to asses short-term 3-6 weeks glycemic control
• urine microalbumin is used to detect very small levels of protein in urine
• RPG 200 mg/dl
• FPG 126 mg/dl
• OGTT 200 mg/dl
• HbA1c 6.5%
• Cuprous Ions + Arsenomolybdate = Arsenomolybdenum Blue (nelson somogyl method)
• Cuprous Ions + Phosphomolybdate = Phosphomolybdenum Blue (Folin Wu Method)
• Cuprous Ions + Neocuproine = Yellow or Yellow Orange (Neocuproine Method)