1. Sterilise petri dish and agar by heating
2. pass inoculation loop through a Bunsen burner flame to sterilise
3. use loop to evenly spread bacteria onto agar plate
4. lift lid or petri dish as little as possible to reduce entry of microbes from air
5. Place antibiotic disks on agar using sterile tweezers
6. lightly tape the sides of the petri dish
7. Incubate for 48 hours upside down at 25 degrees Celsius
8. Measure zones of inhibition