Rate of Reaction

• If the temperature increases, the rate increases until it becomes too hot breaking some of the bonds holding the enzyme together. This denatures it as the active site changes shape.
• If the pH is too high or low, it interferes with the bonds holding the enzyme together changing the shape of the active shape causing it to become denatured.
• The higher the substrate concentration, the faster the rate of reaction. It is more likely for a substrate to bind to an active site. However, increasing the concentration after a certain point has no effect as all active sites are full.
• At the optimum pH or temperature, the rate of reaction is at its best and quickest.
• Mean Rate of Enzyme Activity = Amount of Substrate Used / Time or

  Amount of Product Formed / Time