Building blocks of all living things, have organelles which are specialized structures that carry out specific functions within the cell
Hierarchical organization
From the smallest level (organelle) to the largest level (organism), ensures each level contributes to the overall functioning of the organism
Metabolism
Chemical reactions in an organism, includes cellular respiration and photosynthesis
Cellular respiration
Converts food into energy to carry out activities and maintain life functions
Photosynthesis
Process through which plants convert sunlight, water, and carbon dioxide into glucose and oxygen
Homeostasis
Ability of organisms to maintain a stable internal environment despite changes in the external environment
Heredity
All living things possess DNA which carries genetic information and determines traits and characteristics
Reproduction
Asexual
Sexual
Asexual reproduction
Production of genetically identical offspring from a single parent
Sexual reproduction
Fusion of male (sperm cell) and female (egg cell) reproduction cells (gametes), resulting in offspring with genetic variation
Growth and development
All living things exhibit growth throughout their life cycles, increase in size and complexity, includes embryonic development
Response to stimuli
Living things can respond to internal or external factors that trigger a response, examples include movement, changes in heart rate, or adapting to environmental conditions
Microscopes are essential tools for studying cells and other microscopic organisms, they allow observation and analysis of structures too small to see with the eye
Using a microscope
Turn on light, start at lowest magnification, adjust coarse and fine adjust knobs to bring specimen into focus, switch to higher magnification only using fine adjustment
Total magnification
Magnification of eyepiece (ocular lens) multiplied by magnification of objective lens
Calculating specimen size
Measure field of view diameter using grid slide, convert to micrometers, estimate how many specimens fit across diameter, use formula: FOV (micrometer) / Est. # of specimens fit across diameter
Preparing wet mount slide
Place specimen on slide, add drop of water, cover gently with coverslip to avoid air bubbles, prevents specimen from drying out
Staining
Used to enhance visibility of certain structures within the specimen, examples include methylene blue or iodine
Organelles
Nucleus
Cell membrane
Cytoplasm
Mitochondria
Ribosomes
Cell wall
Chloroplasts
Vacuole
Levels of organization
Organism
Tissue
Organ
Cell
Organelle
Photosynthesis
Occurs in chloroplasts, converts sunlight, water, and carbon dioxide into glucose and oxygen
Cellular respiration
Occurs in mitochondria, converts oxygen and glucose into energy (ATP) and expels carbon dioxide as waste
Products of photosynthesis
Glucose
Oxygen
Products of cellular respiration
Carbon dioxide
Water
Heredity
Passing of genetic material from parent to offspring, ensuring continuity of species
DNA
Carries genetic information that determines traits and characteristics
Sexual reproduction
Fusion of male and female gametes, resulting in offspring with genetic variation
Advantages of asexual reproduction
Faster
More efficient
Disadvantages of asexual reproduction
Lack of genetic variation
Susceptibility to disease
Advantages of sexual reproduction
Genetic variation
Adaptability
Disadvantages of sexual reproduction
Slower
More complex
The cells have identical genetic instructions, but different parts of these instructions are being expressed in each cell
Growth and development
Increase in size and complexity of living organisms, guided by DNA instructions, results in specialized cells
Example of a specialized cell in the human body: muscle cell
Parts of a microscope
Eyepiece
Body tube
Objective lens
Stage
Diaphragm
Nosepiece
Coarse adjust knob
Fine adjust knob
Light source
Base
Arm
Clip
Measuring field of view (FOV) and calculating specimen size
Measure FOV diameter using grid slide, convert to micrometers, estimate number of specimens that fit across diameter, use formula: FOV (micrometer) / Est. # of specimens fit across diameter
The laboratory technique shown is preparing a wet mount slide
The stain used to stain cheek cells was methylene blue
The cover slip should be lowered at an angle to avoid air bubbles