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Biology research design and analysis
Data
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Nailea Estrada
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Cards (46)
Nominal
/
Categorical
Data
Classes
categories
ex- flower color
Ranked
/
Ordinal
Data
Integers
that reflect a
heirarchy
ex- birth order
Measurement Data
Discrete
numerical
and
fixed
in nature
ex- number of flower petals
Continous Data
any number of values between
two
points
ex - height, length, mass
Observational Studies
compares variables measured from different
conditions
Comparative Studies
Independent
variable varies within a system
ex-
soil
,
temp
tests
hypothesis
limits
: small sample size
Perturbation/Response studies
Utilizes
natural
conditions
Large
scale disturbances
ex-
natural
disasters
Manipulative
impose treatments
observe
responses to treatments
I.V-
predictor
D.V -
response
Deductive Studies
specifies
values
for variables or
conditions
Sources of Varition
random error variation
treatment
effects
experimental
artifacts
How to minimize randon error variation and experimental artifacts
high degree
of accruacy and precison
effective
controls
absence of
bias
Bias
can occur at
sampling
, treatment application, measurement
protocol
Replicates
Controls and quantifies random variation
each replicate must be
indepenednt
increase
replicates means more
population
Randomization
eliminates sources of
bias
insures
independence
of data
Features of a good research design
random
variation
high degree of
accuracy
and precision
abscene of
bias
Descriptive
statisistics
displayed as
tables
and
figures
Inferential Statisitcs
draws
conclusions
and makes
predictions
about population
Central tendency
mean
median
mode
Dispersion
scattering of values of a frequency distribution
variance
standard deviation
Confidence Interval
can interpret as: We are
95
% confident that the true
population
mean is between these values
as
alpha
gets smller interval gets
larger
Confidence Interval Depends on
Sample
mean
SE
Level of
confidence
Parametric Statisitcs Assumptions
data are
normally
distributed/
Independent
count
data
Equal
variance
Regression
simple linear model
dependent variable is distributed about the
line
describes the
relationship
b/w the dependent and independent variable
Regression
equation:
Yi=a+bx
where:
yi=
dependent
variable
Bx=
slope
a=
y-intercept
X=
independent
variable
Correlation
No
cause
/ effect
describes
the
linear
relationship b/w two variables
no
line
drawn just
dots
computes "r"
Regression
looks at
cause
/
effects
relationships
computes "
r^2
" or
R^2
manipulates
IV
Finds
line
of
best fit
Regression Line how to interpret Y intercept
If B=0 then there is
no relationship
b/w x and y
--> X does not affect Y
If B≠0 then there is a
relationship
b/w X and
Y
--> X does affect
Y
Deviations
deviations
about the
mean
eq: Xi-x̄
sum of square deviations eq: ∑ (
x-x̄
)^
2
Residuals
deviations
about the
Line
eq:
Yi-Ŷ
sum
of
squares
residuals eq: ∑(yi-Ŷ)^2
where Ŷ becomes
1/2
b/c x becomes
y
Covarinace
part of the
variance
of one variable (y) depends upon the
variance
of another variable (x)
how much total variation is due to X and how much is
unexplained
Line of best fit Regression model
results in smallest ss residuals
not due to
X
r^2 coefficient of determination
ss
regression
/ ss
total
=1
Total variation =
Variation Regression
+ variation of residuals value cant be >
1
higher r^2
=
more
variation
R^2
coefficent
of determination
no
critical
value
R^2= 1 matches
line
and
data
points
R^2=
0
there are no
linear
relationship b/w X and Y
Overview correlation
correlation≠
causation
computes "r"
no cause or effect relationships
Overview Regression
Linear
Regression quantifes goodness of fit
computes
R^2
or
r^2
reports as
ANOVA
Fn,d,=
crit
,
P-value
X^2
∑(
O-E
)^
2
--------
E
X^2 with two groups
includes
yates
correction
∑(|
O-E
|
-0.5
)^2/E
X^2 assumptions
data are
count
data
independence
of data
Advantages of X^2
does not assume
normal
distribution
variance
is not an issue
How to report X^2
X^2 df=
value
,
p-value
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