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MicroPara
sterilization and disinfection
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essential to prevent the transmission of disease
control of microorganisms
controlled by means of chemical and physical agents
microorganisms
external factors that influences the growth of bacteria
physical agents
use to prevent or control the growth of bacteria
chemical agents
destroys bacteria/all living organisms
sterilization
eliminates microorganisms (not all)/cleans surfaces
disinfection
removal of dangerous substances on an area making it safe to hande
decontamination
used to disinfect areas and are too toxic for human tissues
disinfectant
used to kill growth of microbes that are safe to use on human tissues
antiseptic
an agent that reduces microbial numbers on an area
sanitizer
an action that kills all microorganisms
cidal
inhibits the growth of microorganisms
static
factors in evaluating methods of controlling microorganisms
concentration
,
intensity
,
length of exposure
,
temperature
,
number of microorganisms
,
organism itself
,
nature of the material
intensity of the heat present
temperature
microbes have what temp for growth?
maximum
,
optimum
and
minimum
it is static in action and slows the what?
below the minimum
,
metabolism
cidal in action that denature enzymes
above the maximum
it is very common in handling microorganisms
temperature
it can be killed at 50-70 degrees per Celsius with what heat
vegetative microorganisms
,
moist heat
resistant to heat and prolonged exposure is the only way to kill these bacteria
bacterial endospores
it can be either applied as dry or moist heat
high temperature
heat that destroys microbes by irreversible denaturation and is common in the process of sterilization with its ability to penetrate microbial cells
moist heat
it could also melt the what in the cytoplasmic membrane
lipids
employs heat under pressure where water temperature boils at 120 degrees Celsius
autoclaving
water boils at what certain temperature
100 degrees Celsius
kills vegetative cells after 10 mins of exposure but certain viruses like hepatitis will survive for at least 30 mins
boiling water
kills microbes through protein oxidation rather than protein coagulation
dry heat
microbial ovens that employ very high dry temp and is used for sterilizing glass ware
hot air sterilization
used to destroy disposable or expendable materials by burning
incineration
mild heating of milk or other material to reduce the possible spoilage attached to it
pasteurization
proposed the process of pasteurization
louis pasteur
inhibits microbial growth by slowing metabolism
low temperature
what temp does refrigeration keeps food fresh for a few days?
5 degrees Celsius
temp at which food is kept fresh for several months in a freezer
-5 degrees Celsius
has a static effect on a microbe that also inhibits the action of microbial enzymes; known also as drying
desiccation
removal of moisture
desiccation
applied pressure to a solution to halt flow of solvent molecules
osmotic pressure
water tends to flow in what in the osmotic pressure?
semipermeable membrane
concentration of the dissolved material is higher inside the cell than outside the cell
hypotonic environment
greater degree of tension
hypernoticity
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