sterilization and disinfection

Cards (49)

  • essential to prevent the transmission of disease
    control of microorganisms
  • controlled by means of chemical and physical agents
    microorganisms
  • external factors that influences the growth of bacteria
    physical agents
  • use to prevent or control the growth of bacteria
    chemical agents
  • destroys bacteria/all living organisms
    sterilization
  • eliminates microorganisms (not all)/cleans surfaces
    disinfection
  • removal of dangerous substances on an area making it safe to hande
    decontamination
  • used to disinfect areas and are too toxic for human tissues
    disinfectant
  • used to kill growth of microbes that are safe to use on human tissues
    antiseptic
  • an agent that reduces microbial numbers on an area
    sanitizer
  • an action that kills all microorganisms
    cidal
  • inhibits the growth of microorganisms
    static
  • factors in evaluating methods of controlling microorganisms
    concentration, intensity, length of exposure, temperature, number of microorganisms, organism itself, nature of the material
  • intensity of the heat present
    temperature
  • microbes have what temp for growth?
    maximum, optimum and minimum
  • it is static in action and slows the what?
    below the minimum, metabolism
  • cidal in action that denature enzymes
    above the maximum
  • it is very common in handling microorganisms
    temperature
  • it can be killed at 50-70 degrees per Celsius with what heat
    vegetative microorganisms, moist heat
  • resistant to heat and prolonged exposure is the only way to kill these bacteria
    bacterial endospores
  • it can be either applied as dry or moist heat
    high temperature
  • heat that destroys microbes by irreversible denaturation and is common in the process of sterilization with its ability to penetrate microbial cells
    moist heat
  • it could also melt the what in the cytoplasmic membrane
    lipids
  • employs heat under pressure where water temperature boils at 120 degrees Celsius
    autoclaving
  • water boils at what certain temperature
    100 degrees Celsius
  • kills vegetative cells after 10 mins of exposure but certain viruses like hepatitis will survive for at least 30 mins
    boiling water
  • kills microbes through protein oxidation rather than protein coagulation
    dry heat
  • microbial ovens that employ very high dry temp and is used for sterilizing glass ware
    hot air sterilization
  • used to destroy disposable or expendable materials by burning
    incineration
  • mild heating of milk or other material to reduce the possible spoilage attached to it
    pasteurization
  • proposed the process of pasteurization
    louis pasteur
  • inhibits microbial growth by slowing metabolism
    low temperature
  • what temp does refrigeration keeps food fresh for a few days?
    5 degrees Celsius
  • temp at which food is kept fresh for several months in a freezer
    -5 degrees Celsius
  • has a static effect on a microbe that also inhibits the action of microbial enzymes; known also as drying
    desiccation
  • removal of moisture
    desiccation
  • applied pressure to a solution to halt flow of solvent molecules
    osmotic pressure
  • water tends to flow in what in the osmotic pressure?
    semipermeable membrane
  • concentration of the dissolved material is higher inside the cell than outside the cell
    hypotonic environment
  • greater degree of tension
    hypernoticity