Spectrophotometer

• spectrophotometer

  A) light source

  B) entrance slit

  C) monochromator

  D) exit slit

  E) sample cuvet

  F) PM tube

  G) A/D

  H) display

  
• parts of spectrophotometer

  • light source must be able to transmit visible light and ultraviolet light
• parts of spectrophotometer

  • entrance slit - narrows beam of light heading towards sample
• parts of spectrophotometer

  • monochromator - separates white light into individual wavelengths
• a monochromator can be a prism or a diffraction grating
• an exit slit allows only the wavelength of light we are looking for to pass through
• incident light is the light hitting the sample
• the transmitted light is the light that is not absorbed into the sample; light that gets through
• photomultiplier or photodetector - will detect transmitted light
• PM tube sends signal to a/d - analog to digital converter
• spectrophotometer

  A) light source

  B) entrance slit

  C) monochromator

  D) exit slit

  E) cuvette

  F) PM tube

  G) readout device