Objective lenses: MOST IMPORTANT optical components of the microscope because they produce the primary image magnification.
Objective lenses produces what?
primary image magnification
Objective lenses: ENLARGE and PROJECT the illuminated image of the object toward the eyepiece.
What are the 4 types of objective lenses?
Scanner, LPO, HPO, and Oil Immersion
What is the magnification of Scanner?
4x
What is the magnification of LPO?
10x
What is the magnification of HPO?
40x
What is the magnification of Oil Immersion?
100x
What is the TOTAL magnification of Scanner?
40x
What is the TOTAL magnification of LPO?
100x
What is the TOTAL magnification of HPO?
400x
What is the TOTAL magnification of Oil Immersion?
1000x
Red: color of scanner
Yellow: color of LPO
Blue: color of HPO
White: color of Oil Immersion
What is the commonly used oil for oil immersion?
Cedar wood oil
What is the magnifying power of Oculars or Eyepieces?
10x
Monocular: only one eyepiece
Binocular: two eyepieces
Diopter Adjustment Knob: Knob on the oculars can be rotated to compensate for variation in vision between the operator's eyes.
Coarse Adjustment knob: Used to move the stage UP or DOWN very rapidly
Fine Adjustment knob: It is used to move the stage UP or DOWN very SLOWLY. The stage covers a very small distance on each rotation to SHARPEN the image.