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Biology
Module 2 – Foundations in Biology
Chromatography PAG
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LoudOwl3678
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Cards (4)
Why must the origin line be drawn in pencil, not pen?
Ink
from the pen would
dissolve
in the
solvent
and
run
(leads to
inaccurate
results)
Why should you draw a line where the solvent reached immediately?
Solvent
evaporates rapidly
so the
end position
is not
visible
shortly after the experiment.
Why should you measure the rf value from the middle of the pigment mark?
The pigment mark is
spread out
, so measuring from the middle position
standardises
the measurements you are taking to allow for comparisons
Why should you make sure your chromatography paper is vertical and straight?
So the pigments move straight
up
the paper to avoid them running off the
side
of the paper and/or being
washed
off.