1. Spray the bench you are working on with disinfectant then wipe dry with paper towels
2. On the bottom of the agar plate (not the lid) mark with a wax pencil / permanent marker: 3 segments, a dot in the middle of each segment, your initials, date, name of bacteria
3. Wash your hands with antibacterial handwash
4. Place the different antiseptics onto different filter paper discs
5. Lift the lid of the agar plate at an angle carefully and use forceps to place each filter paper disc onto the dots. Note down the antiseptic applied to each zone
6. Tape the lid onto the agar plate securely, but loosely enough that oxygen can still reach the bacteria
7. Place the agar plate in the incubator at 25°C for 48 hours
8. Measure the diameter of the clear zones after 48 hours using a ruler. Take a second measurement at 90 degrees from your first measurement and take a mean for the diameter. Do not remove the lid when taking measurements
9. Record the results in a table
