Cell Wall: Gram positive - The thick cell wall is composed of peptidoglycan and teichoic acids may also be present. Gram negative - The outer membrane is composed of lipids, proteins, LPS, and thin inner peptidoglycan layer.
Shape: Gram positive - Spherical, rod-shaped or filamentous. Gram negative - Spherical, oval, straight or curved rods, helical or filamentous.
Metabolism: Gram positive - Chemoorganoheterotrophic. Gram negative - Phototrophic, Chemolithoautotrophic, Chemoorganoheterotrophic.
Teichoic Acid: Gram positive - Present. Gram negative - Absent.
Endospore: Gram positive - Present in some. Gram negative - Absent.
Periplasmic Space: Gram positive - Absent. Gram negative - Present.
Flagellar Structure: Gram positive - 2 rings in the basal body. Gram negative - 4 rings in the basal body.
Resistance to physical disruption: Gram positive - High. Gram negative - Low.
Resistance to lysozyme: Gram positive - Low. Gram negative - High.
Inhibition by basic dye: Gram positive - High. Gram negative - Low.
Reproduction: Gram positive and Gram negative - Binary fission.