1) put a drop of iodine solution on spotting tile
2) put a water beaker on top of a Bunsen burner, heat until 35 degrees. keep temp constant
3) use syringe to add 1cm of amylase solution to 1cm of a buffer solution with pH 5.
4) Put tube into water beaker for 5 minutes
5) use syringe to add 5cm of a starch solution to boiling tube.
6) mix contents of boiling tube and start a stop watch.
7) starting next to the control variable, use continuous sampling add the solution into the iodine solution every 30 seconds
8) repeat with buffers of different pH