Proteins acting as catalysts, made by living cells to speed up biochemical reactions, where the enzyme converts substrates into products
Catalyst
A substance that increases the rate of a chemical reaction but is not changed by the reaction
Rate of reaction
The speed at which a chemical reaction occurs
Substrate
The substance that an enzyme interacts with to create a product
Product
The substance formed as a result of a chemical reaction
Metabolism
All the chemical reactions that occur in living organisms, both intracellular and extracellular
Intracellular
Reactions that happen inside of the cells
Extracellular
Reactions that happen outside of the cells, for example digestion
Enzymes
They are specific, and each enzyme catalyses one specific reaction or group of reactions
Because of enzyme specificity, living organisms have to produce many different enzymes
Catabolism
Reactions that break down larger molecules into smaller ones, for example digestion of food
Hydrolysis reaction
The reactions that occur during digestion, used to deconstruct polymers into monomers
Anabolism
Reactions that build up smaller molecules into larger ones, they require energy, for example photosynthesis. Macromolecules are produced from monomers using ATP. They are condensation reactions
Condensation reaction
A reaction where water is produced as a by-product
Active site
The specific region on the surface of the enzyme where the substrate binds to so that the reaction can be catalysed. The shape of the active site is specific so only certain substrates can bind to it
Induced fit hypothesis
When the substrate binds to the active site, the interaction causes them both to change and both their bond angles and bond lengths are altered, chaining the three-dimensional molecular shapes of the substrate and the active site
Enzyme-substrate complex
The temporary association between the enzyme and its substrate during a chemical reaction, where the enzyme binds to the substrate at the active site
If the temperature increases in a liquid
The rate of the substrate-active site collision will increase since it leads to a faster molecular motion
Substrate concentration
The amount of substrate present in a chemical reaction
Denaturation
When changes occur to an enzyme, such as the protein structure being altered by temperature, or chemicals, the enzyme no longer works as a catalyst. When the changes cannot be reversed the enzyme is denatured
Enzyme activity
How well an enzyme works in catalysing a chemical reaction
Optimum
A specific temperature or pH-value where the enzyme activity is the highest. Each enzyme has their own specific different optimums
pH
A measure of the concentration of hydrogen ions, a low pH means high concentration of hydrogen ions. Determines whether a substance is basic or acidic
Polarity
A molecule having a positive and a negative pole
Amino acids
The building blocks of proteins. Consists of one amine group, a carboxyl group, and a side chain
Transition state
A state that the chemical reaction needs to reach before it can be converted into a product. Energy is required for this; it is called activation energy and is used to break bonds in substrate molecules
Activation energy
The energy required to reach the transition state. It is used to break bonds in substrate molecules
Independent variable
The factor that is being investigated; they are varied to see what the effect is
Dependent variable
The result of the experiment, the factor that is being measured. The independent variable affects the dependent variable
Controlled variable
Factors that must be kept constant in an experiment so that they don't affect the results
Adenosine triphosphate (ATP)
A molecule used by cells as a source of energy
Adenine
A component of ATP, a nitrogenous base
Ribose
A sugar molecule found in the structure of ATP
Phosphate, Pi
A chemical group essential for ATP structure and function
Nucleotide
The building block of nucleic acids, like DNA and RNA, consisting of a nitrogenous base, a sugar, and a phosphate group
Water soluble
Able to dissolve in water
Synthesis
The process of combining simpler substances to form more complex ones
Anabolic reaction
A metabolic process that builds larger molecules from smaller ones, requiring energy
Endothermic reaction
A chemical reaction that absorbs heat from its surroundings
Condensation reaction
A chemical reaction in which two molecules combine to form a larger molecule with the elimination of water
Active transport
The movement of molecules across a cell membrane against their concentration gradient, requiring energy