Serial Dilutions

• A serial dilution is the step-by-step dilution of a substance in solution.
• 𝙎𝙀𝙍𝙄𝘼𝙇 𝘿𝙄𝙇𝙐𝙏𝙄𝙊𝙉:

  A) 100%

  B) Stock

  C) 10%

  D) 1%

  E) 0.1%

  F) 1

  G) water

  H) 10%

  I) 9

  J) 1 part 1%

  K) 9

  L) water

  
• Serial dilutions allow you to make solutions with very low concentrations, without having to measure out very small volumes, and this improves accuracy.
• Add 1 part bacteria culture to 9 parts water (to make 10⁻¹ dilution);

  Mix;

  Repeat using 9 parts water and 1 part of 10⁻¹ and 10⁻² dilutions to make the 10⁻³ dilution.

  
• 𝙎𝙀𝙍𝙄𝘼𝙇 𝘿𝙄𝙇𝙐𝙏𝙄𝙊𝙉𝙎 𝙏𝘼𝘽𝙇𝙀:

  𝘝1:

  V1 = (C2 x V2) / C1

  V1 = (0.08 x 20) / 1

  V1 = 1.6 cm3

  𝘞𝘢𝘵𝘦𝘳:

  V2 = V1 + volume of water

  volume of water = V2 - V1

  volume of water = 20 - 1.6

  volume of water = 18.4 cm3

  A) Volume of water

  B) cm3

  C) C1

  D) C2

  E) V2

  F) 1.6cm3

  
• 𝙎𝙀𝙍𝙄𝘼𝙇 𝘿𝙄𝙇𝙐𝙏𝙄𝙊𝙉𝙎 𝙏𝘼𝘽𝙇𝙀:

  𝘝1:

  V1 = (C2 x V2) / C1

  V1 = (0.08 x 20) / 1

  V1 = 1.6 cm3

  𝘞𝘢𝘵𝘦𝘳:

  V2 = V1 + volume of water

  volume of water = V2 - V1

  volume of water = 20 - 1.6

  volume of water = 18.4 cm3

  A) C2

  B) C1

  C) V2

  D) Volume of water

  E) cm3

  F) 1.6cm3

  G) 18.4cm3