Chromatography

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    Created by
    charlie rice

    Cards (11)

      1. Chromatography paper is cut to fit the tank
    • 2. The solvent should be placed in the chromatography tank and the lid attached allowing the solvent to fully saturate the tank
    • 3. The chromatogram should be long enough to extend into the solvent but the solvent should remain below the base line
    • 4. The base line should be drawn in pencil a few cm above the bottom of the paper
    • 5. The solution containing the plant pigment should be spotted om the centre of the pencil time, repeat at least 5 times for a concentrated spot
    • 6. The chromatogram is then suspended into the solvent and securely attached to the lid of the tank
    • 7. the solvent is allowed to run up the chromatogram at least 3/4 of the way before it reaches the top
    • 8. The solvent front is marked in pencil
    • 9. The chromatogram is dried
    • 10. Rf value of each spot is calculated
    • Rf = distance travelled by solute
      ————————————————
      distance traveled by solvent
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