Chromatography

Cards (11)

  • Paper Chromatography: A method of separating dissolved substances based on their solubility in water
  • Soluble: (of a substance) able to be dissolved
  • Insoluble: unable to dissolve in another solvent
  • Solubility: the ability to be dissolved
  • solvent: a liquid that can dissolve other substances, such as water
  • Chromatography works because some substances dissolve in the solvent better than others and travel up the paper further
  • Paper Chromaography:
    A) solvent front
    B) distance travelled by solvent
    C) distance travelled by substance
    D) baseline
  • Rf value = Distance travelled by substance / Distance travelled by solvent
  • The Rf values are used to identify unknown compounds.
  • Stationary phase - paper
    mobile phase - solvent
    result of chromatography experiment - chromatogram
  • molecules with a higher solubility in the solvent (therefore less attracted to the paper) will spend more time in the solvent and will be carried further up the paper