The two cells that surround the stomatal pore and regulate the opening and closing of the pore.
When the leaf has a lot of water, cells will fill and go turgid, opening the stomata to allow gases can be exchanged for photosynthesis
When the leaf has too little water, guard cells lose water and go flaccid, closing the stomata and stopping too much water from escaping
Sensitive to light - close at night to reserve water when gas exchange is not needed (as photosynthesis cannot occur at night)