Dr. Kary Mullis

• Came upon a way to double his test target by giving him how many copies?

  2^1 copies
• Repeating N times would yield?

  2^N
• If he repeated the process, the target would double again, giving how many copies?

  2^2 or 4 copies
• After N doublings, he would have how many copies in his target?

  2^N copies
• If N=30 or 40, what will happen?

  There would be millions of copies.
• The would be a million copies by adding a/an?

  Oligonucleotide primer with the 4 dNTPs and DNA polymerase in a chain reaction.
• Dr. Kary Mullis was a 1993 Nobel Prize Winner in?

  Chemistry for the Polymerase Chain Reaction.
• The first successful ampliction was a short fragment of the?

  Escherichia coli plasmid, pBR322
• The first paper describing a practical application, the amplification of beta-globin and analysis for diagnosis of ptients with sicle cell anemia was published when?

  2 years later
• What was the enzyme Dr. Kary Mullie used to drive the replication of DNA and once it started, the replicationcontinued in a chain reaction?

  DNA Polymerase